Rapid isolation method for lipopolysaccharide and lipid A from gram-negative bacteria.

Rapid plasmid DNA isolation from mucoid gram-negative bacteria.

Exopolysaccharides interfere with the isolation and characterization of plasmid DNA from gram-negative bacteria. To repress capsular polysaccharide production, bacteria were cultured in medium containing bismuth nitrate and sodium salicylate. Rapid removal of other contaminating bacterial surface components was achieved by mild acidic zwitterionic detergent extraction. After treatment, bacteria...

Simple and rapid method for isolation of RNA from gram-negative bacteria.

The profile of pathogenic bacteria isolated from dental plaque-induced gingivitis

Gingivitis is an inflammatory destructive disease mainly caused by microbial plaques. Gram-positive and gram-negative bacilli or cocci have been isolated from dental plaque and gingivitis. This study was going to isolate and characterize the pathogenic bacteria from gingival of 100 patients with oral gingivitis. Enriched and specific media used for isolation of bacteria. Identification of isola...

Investigating the Antimicrobial Efficacy of Liposomal Vancomycin in Gram-positive and Gram-negative bacteria- A Preliminary Mechanistic Study

Outer membrane of Gram-negative bacteria is a permeability barrier to many antibacterial agents, including the glycopeptide antibiotics such as vancomycin hydrochloride and as a result these antibiotics are ineffective against Gram negative bacteria. Different strategies have been described to overcome such limitation, including application of nanoparticles, as was shown in our previous studies...

Evolution of gram-negative bacteria: a view from lipid A biosynthesis enzymes

Lipid-A, a complex glycolipid, is the highly immunoreactive endotoxic center of lipopolysaccharide (LPS). It anchors the LPS into the outer membrane of most gram-negative bacteria. Lipid A can be recognized by animal cells, triggers some defense-related responses, and causes gram-negative sepsis. The lipid-A biosynthesis pathway consists of nine enzymatic steps. The enzymes involved are: LpxA, ...